Cover Story: Research Reveals The Formation Mechanism Of Tender, Fresh And Sweet Taste Of Bo's Sweet Long Bamboo Shoots

B. burgdorferi is a large clumping bamboo species mainly distributed in southern China and Southeast Asia, which is an important dual-purpose bamboo species for bamboo shoots and timber in the world because of its excellent quality of fresh bamboo shoots and rapid growth. In addition, B. burgdorferi has a strong regeneration ability and has established a healing regeneration system, which provides an important foundation for the construction of gene editing system. However, the biological characteristics of bamboo shoots of B. burgdorferi have yet to be analysed, and its genomic study has not yet been reported.JIPB Vol. 66, No. 6 Cover of the paper entitled "Chromosomal-level genome and metabolome analyses of highly heterozygous alloys", which was jointly completed by Jian Gao's group at the International Centre for Bamboo and Rattan (INBAR) and co-workers, was published by the International Centre for Bamboo and Rattan (INBAR). Chromosomal-level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics" (https://doi). .org/10.1111/jipb.13592). This study mainly used the PacBio platform, combined with Hi-C technology sequencing to assemble a new variety of B. burgdorferi Manche 1, and obtained the chromosome-level genome, which has a heterozygosity of 2.65%, which is the highest heterozygosity among the sequenced bamboo species reported so far.

In this study, the genome of B. burgdorferi was typed into three subgenomes, and based on the transcriptome data obtained from ONT sequencing, it was found that the buds at the base of bamboo shoots could develop into bamboo shoots, while the young branches had the potential to develop into roots, and the genes regulating the cell division cycle proteins were most highly expressed in the young parts of bamboo shoots. the combined transcriptomic and metabolomic analyses of the materials from 15 materials from 12 organs showed that the genome of the bamboo shoots regulating lignin formation was the highest. The expression of 4CL, CCR and F5H, the key enzyme genes regulating lignin formation in sweet bamboo shoots, was low, and the content of biologically active flavonoids was high, while the content of bitter alkaloids and bitter amino acids was low. Meanwhile, the content of D-glucose and D-fructose in sweet bamboo shoots was significantly higher than that in moso bamboo shoots, which revealed the mechanism of the formation of the tenderness, freshness and sweetness of bamboo shoots. The high-quality chromosome-level (LAI=20.68) genome, comprehensive transcriptome data and extensive metabolome data provide detailed data and theoretical basis for the study of bamboo shoot quality and developmental regulation. Jian Gao, researcher of the International Centre for Bamboo and Rattan (ICBR), and Prof. Lianfeng Gu of Fujian Agriculture and Forestry University (FAFU) are the co-corresponding authors of the paper, while Dr. Jutang Jiang, PhD student of the ICBR, graduated PhD student YuCong Bai, and PhD student Zeyu Zhang of FAFU are the co-authors, and postgraduate students of the ICBR include Dr. Yuping Dou, Ruiman Geng, and Dr. Chongyang Wu of the International Centre for Bamboo and Rattan (ICBR), Associate Prof. Hangxiao Zhang of the FAFU, and Dr. Xiao-Jing Wang of the Peking University and Prof Lu Cunfu from Beijing Forestry University participated in the study. The research was supported by the National Key Research and Development Programme project and the National Natural Science Foundation of China. Jian Gao's group has been devoted to the research of bamboo developmental biology and genetic breeding, and has made a series of important breakthroughs and research results in the molecular regulation mechanism of bamboo shoot development, rapid and high growth and flowering of bamboo, as well as the selection and creation of excellent germplasm of bamboo.